Associate Professor of Chemistry & Biochemistry; Concurrent Associate Professor of Physics
450 Stepan Chemistry Hall
Functional Dynamics of Proteins and Ligands
The life of the cell depends on protein molecules - nanometer-sized "molecular machines" - that serve the vast array of functions supporting cell maintenance and growth. Historically, explanations of protein function emphasized specific 3-d structures, thereby fostering a static view of proteins. Yet, many proteins have substantial regions of flexibility involved in their functional mechanisms, and their number continues to increase. It is now clear that a static view is too limiting. To explain protein function and evolution, we need to acknowledge their intrinsic flexibility and understand the atomic-level determinants of their functional dynamics. From a physics standpoint, proteins give an opportunity to understand the behavior of complex, dynamical systems.
The over-arching goal of our lab is a predictive understanding of how conformational dynamics affects the evolution of protein function. Our main methods are multi-dimensional liquid-state NMR spectroscopy and computation. Specific research areas include: i) exploring the role of conformational dynamics in allosteric signaling; ii) defining flexibility-function correlations for proteins conferring antibiotic resistance; and iii) development of improved NMR methods for establishing flexibility-activity relationships in iterative inhibitor design. These research areas are relevant to deepening our understanding of the physics of proteins and how they evolve, particularly in the context of drug resistance.
- "Negative Regulation of Peptidyl-Prolyl Isomerase Activity by Interdomain Contact in Human Pin1" Wang, X.; Mahoney, B.J.; Zhang, M.; Zintsmaster, J.S.; Peng, J.W. Structure 2015, 23(12), 2224-2233.
- "Investigating dynamic interdomain allostery in Pin1" Peng, J.W. Biophys. Rev. 2015, 7(2), 239-249.
- "Investigation of Signal Transduction Routes within the Sensor/Transducer Protein BlaR1 of Staphylococcus aureus" Staude, M.W.; Frederick, T.E.; Natarajan, S.V.; Wilson, B.D.; Tanner, C.E.; Ruggiero,S.T.; Mobashery, S.; Peng, J.W. Biochemistry 2015, 54(8), 1600-1610.
- "Revealing Cell-Surface Intramolecular Interactions in the BlaR1 Protein of Methicillin-Resistant Staphylococcus aureus by NMR Spectroscopy" Frederick, T.E.; Wilson, B.D.; Cha, J.; Mobashery, S.; Peng, J.W. Biochemistry 2014, 53(1), 10-12.